Mean concentration of the characterization parameters of raw and treated wastewaters and comparative analysis with the CONSEMA/RS /06 and UWTD. When we observe the resolution of CONSEMA (/) concerning the liquid effluent discharge conditions in receiving water body, they may. More speciļ¬cally, water (CONSEMA No. /) [12] and the recommendations of Dos balance by measurement of water consumption and of the Santos [13].

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Evaluation of phytotoxicity of municipal landfill leachate before and after biological treatment. In the present study, leachate toxicity of a municipal solid waste landfill located in the Sinos River Valley region southern Brazil was evaluated using plant bioassays. Leachate toxicity was assessed by analysis of seed germination and root elongation of lettuce Lactuca sativa L. Bioassays were performed by exposing the seeds of L.

The levels of metals detected in both samples of leachate were low, and raw leachate showed high values for ammoniacal nitrogen and total Kjeldahl nitrogen. There is a reduction in the values of several physicochemical parameters, which demonstrates the efficiency of the treatment. However, the responses of these two plant species were different. Root elongation was significantly lower in A.

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Seed germination showed no difference across the treatments. The results of the study show that plant species respond differently and that municipal solid waste landfill leachate show phytotoxicity, even after biological treatment.

Os resultados indicam fitotoxicidade do chorume em L. O crescimento radicular foi significativamente inferior em A. One of the main environmental problems caused by the urbanisation in cities concerns the disposal of municipal solid waste Cknsema et al. Waste disposal in landfills is an increasingly common practice in large cities Abrelpe,and generates large volumes of leachate as the product of waste decomposition and percolation of rainwater.

Leachate is a dark liquid, with unpleasant odour; it is highly toxic and should be handled properly before its release in receiving bodies Baun et al. The most common conventional treatments for cpnsema type of effluent are biological aerobic and anaerobic treatments Renou et al. However, this kind of treatment often presents low efficiency in the case of leachate coming from old landfills, due to reduced biodegradable fraction, presence of high molecular weight substances Conssema et al.

Hazard assessment of landfill leachate is traditionally based on the evaluations of individual conseka identified through chemical analysis.

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However, chemical data alone do not allow for the evaluation of toxic effects. Some studies show that leachate can cause deleterious effects on organisms at various trophic levels Bakare and Osibanjo, ; Christensen et al.

Among the various toxicity tests available, the seed germination cosema root elongation is a widely used, quick and accurate phytotoxicity test, showing sensitivity, simplicity, low cost and suitability for unstable chemicals or samples Wang et al. Different model species are used in bioassays, including lettuce Lactuca sativa L. However, in these locations, there is still generation and treatment of leachate, which is consemma released in the water resources. The water quality in the basin is influenced by the direct discharge of domestic and industrial sewage, which have the potential to cause toxic effects on genetic and cellular levels Blume et al.

Although the release of leachate poses an environmental risk, the effectiveness of its treatment and its toxicity in the Sinos River Basin were not assessed.

This study aims to evaluate the phytotoxicity of leachate from a municipal landfill in three plant species, before and after biological treatment. The leachate collection took place during September spring seasonin a municipal solid waste landfill located in a large municipality in the Sinos River Valley region. The landfill is approximately 50 years old and has been disabled, but its pits still generate leachate, which passes through a biological treatment system biodegradation is carried out by microorganisms in a treatment pond.


An aliquot of raw leachate was collected at the input and another aliquot of treated leachate was collected at the output of the biological treatment pond. Immediately after collection, the samples were sent for physicochemical characterisation and the following parameters were determined: Onion bulbs Allium cepa were purchased commercially and kept in a moisture-free location, protected from light.

Prior to the test, roots that were old and dried were removed. In order to stimulate root growth, onions were kept in tap water for a period of 24 hours before exposure. A negative control group was treated with tap water. Five onion bulbs were exposed to each treatment at room temperature and insulated from direct sunlight for a period of 48 hours. At the end of the exposure period, the length of the three longest roots in each bulb was measured.

The average length of the roots in each treatment was calculated and compared to the growth in the control group. Toxicity was assessed by inhibition of root growth in the leachate samples, when compared to the control group. The germination and root growth tests were performed using Lactuca sativa and Eruca sativa seeds obtained from local retailers. Protocol consisted of exposure of the Lactuca sativa and Eruca sativa seeds in Petri dishes prepared with a filter paper and 5 mL of the raw and treated leachate samples, and tap water for the control group.

For each treatment, fifty seeds were exposed in one Petri dish. Time of exposure was hours, at room temperature and insulated from direct light. At the end of the treatment, germination rate and root growth were estimated.

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Seed germination was analysed by the Chi-square test. The results of the physicochemical analyses are presented cobsema Table 1. Among the parameters evaluated in the sample of raw leachate, the nitrogen compounds in the series, ammoniacal nitrogen and total Kjeldahl nitrogen, showed very high values.

The other parameters, with the exception of manganese, were found to be within the emission limits established in the Consema Resolution No. A change was observed in the characteristics of the leachate after the biological treatment. There is a reduction in the values of conductivity, BOD 5COD, chlorides, total phosphorus, turbidity and metals, which demonstrates the efficiency of the treatment.

In contrast, an increase in the concentration of nitrite and nitrate was observed. Total Kjeldahl Nitrogen parameter was still found to be outside the limit set by state law The results of the toxicity assessment in A. The results of seed germination and root growth in Lactuca sativa and Eruca sativa are shown in Table 3. The exposure of L. The same effect was observed in E. The conseam of landfill leachate after treatment, in order to ensure safe levels for its disposal in the environment, can be 006 through a series of physicochemical parameters Barker and Stuckey, ; Baun and Christensen, However, none of these parameters alone is able to determine whether the sample may have toxic effects on aquatic biota Rizzo, In this sense, the bioassays are useful tools for assessing the risks to the biota posed by pollutants Knie and Lopes, In southern Brazil, studies assessing the toxicity of landfill leachates are still scarce Bortolotto et al.

Germination and root growth tests allow for an assessment of acute toxicity, both of a pure cosnema and of a complex mixture Torres et al. Toxicity tests based on seed germination and root growth have been proposed by government agencies as part of the evaluation of the potential for contamination of waste and effluents disposed into the environment USEPA, Among the possible environmental contaminants, ammonium, volatile organic acid, heavy metal and salt contents can cause harmful effects on plant development, by inhibiting seed germination or root growth Varnero et al.


In this study, the seed germination parameter was considered to be less sensitive to the toxicity of the leachate. Similar results have also been obtained by other authors Kapustka et al. In this aspect, substances considered to be toxic seem to not directly affect the process of ontogeny of the seed, in the transition from heterotrophic to autotrophic metabolism. The evaluation of root growth indicates that Lactuca sativa and Allium cepa are sensitive to the toxic effects of substances present both in raw and treated leachates.

Evaluation of phytotoxicity of municipal landfill leachate before and after biological treatment

Raw leachate was found to be toxic only for L. Studies have found that conwema lettuce seed is very sensitive to the presence of metals and organic compounds, which makes it suitable for testing the toxicity of effluents Wang, ; Toussaint et al. The inhibition of L. In contrast, after biological treatment, the leachate was found to be toxic only for A. The reason for this variable response on the conaema species could be attributed to inherent differences between the species, such as tissue permeability, differential uptake and metabolism Cutillo et al.

The physicochemical analyses indicate that the leachate presents most parameters within the boundaries of what is acceptable by law, except for nitrogen compounds. However, it is worth noting that some parameters detected in high concentrations in this study, such as chloride, conesma, nitrite and conductivity, consem no legal limits for disposal. The levels of metals detected in both samples of leachate were low, thus indicating a typical domestic waste landfill Christensen et al.

Some results of this study cast doubt on the apparent consfma of biological treatment, which is evidenced only when considering the parameters contained in the legislation.

Furthermore, the phytotoxicity tests showed that toxicity also occurs after conventional biological treatment, thus corroborating the results found by other studies on the toxicity of treated effluents Oral et al. This occurs because some toxic chemicals will not undergo degradation during wastewater treatment, owing to a high degree of persistence. Moreover, waste treatment does not always reduce the toxicity of an effluent and, in some instances, may increase toxicity Claxton et al.

This study has shown that the higher plants species L. Regarding the toxicity consdma leachate, additional knowledge is required in order to address the potential toxicity of untreated leachate and the efficiency of treatment technology considering the influence of environmental conditions on the dynamics of the decomposition processes that take place in the landfill. Cohsema methods for the examination of water and wastewater. Effect of simulated leachate on chromossomes and mitosis on roots of L.

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Primary treatment of anaerobic landfill leachate using activated carbon and limestone: