La propagación de plantas, a través de la embriogénesis somática, Tesis presentada en opción al grado científico de Master en Biotecnología Vegetal. UCLV. Embriogénesis somática en papaya cultivar ‘Maradol Roja’: una alternativa para either to produce a high number of plants (high quality vegetal material to be. La regeneración de plantas de cacao mediante embriogénesis somática se ha El manejo del material vegetal se llevó a cabo en cámara de flujo laminar.

Author: Vojinn Gardahn
Country: Trinidad & Tobago
Language: English (Spanish)
Genre: Career
Published (Last): 24 February 2014
Pages: 397
PDF File Size: 1.82 Mb
ePub File Size: 19.13 Mb
ISBN: 238-1-36251-866-8
Downloads: 6648
Price: Free* [*Free Regsitration Required]
Uploader: Vijora

There are also few reports on the obtainment of secondary multiplication from primary somatic embryos, as a key phase to generate higher number of plants in SE. Dentro de las vias de propagacion in vitro de plantas, la embriogenesis somatica ofrece posibilidades de obtener volumenes de produccion superiores en un menor periodo de tiempo y a un costo mas bajo, lo cual la convierte en un metodo potencialmente mas eficiente que la regeneracion via organogenesis.

Red Maradol plants using zeolite as support. Sin embargo solo los embriones del clon BIOB alcanzaron la etapa cotiledonar.

Topicos de La Embriogenesis Somatica Vegetal

Photoauto- ,Photohetero-and Photomixotrophic in vitro propagation of papaya Carica papaya L. Somatic embryogenesis from integument perisperm cultures of coffee. In this sense, two or three seeds have to be planted per planting spot in order to generate a full hermaphrodite plantation, also embrioyenesis to eliminate the other two undesired phenotypes at flowering.

Plantation Research and Development, pp: Indirect somatic embryogenesis and morphohistological analysis in Capsicum chinense. The methodology developed was supported by histology and electron microscopy studies, and provides a consistent tool to increase the efficiency of the propagation process.

Las plantas de cm de altura se adaptaron a condiciones ex vitro sin necesidad de un subcultivo previo en el medio de mantenimiento como lo describe el protocolo original de Fontanel et al. Phloroglucinol in plant tissue culture. Journal of Virological Methods, Another alternative considers the potential introduction of biologically active substances of national production and available for crop improvement in the in vitro regeneration process of papaya, in order to improve rooting in vitrowhile substituting the import of equivalent foreign products.


The embryos went through four phases; Induction, Formation, Maduration and Mantenimiento which corresponded each one with different media culture. Photoautotrophic micropropagation-environmental control for smbriogenesis photosynthesis. Un total de 67 plantas fueron usadas para este experimento. Ministerio de la Agricultura.

Here we report the establishment of a methodology for plant regeneration via somatic embryogenesis SE from immature zygotic embryos, what supports high survival and high genetic homogeneity of the regenerated plants as determined by AFLP.

Use of secondary somatic embryos promotes genetic fidelity in cryopreservation of cocoa Theobroma cacao L.

Advances in cocoa Theobroma cacao propagation by somatic embryogenesis. Respecto a este evento, Quiroz et al. Vegetative propagation of Quercus suber L. So far, there was found a single report on the papaya tissue culture in vitro by using photoautotrophic conditions [9], but no details were referred about the most critical phase: Biotechnology in Agriculture and Forestry Plant Cell Tissue Organ Cult, Estos fueron procesados siguiendo el protocolo de Maximova et al.

Variation of plants derived from indirect somatic embryogenesis in cotyledon explants of papaya.

Revista Colombiana de Biotecnología

United Nations Conference on Trade and Development. Plant Science This embrkogenesis also provided the first report, up to our knowledge, on the use of zeolite as substrate for the cultivation of shoots in vitro, in vdgetal with increased ventilation as it was carried out. Biologia65 3: Esto es coherente con los resultados obtenidos por Traore et al.


The high embriogendsis value of papaya is the basis for a variety of food and medicinal applications [1]. Other difficulties come from the influence of environmental factors on the resulting plants, which influence on the growth, development and morphogenesis of shoots and plants generated in vitro mainly during the trophic phase. En este ensayo se evaluaron como explantes dos estados de desarrollo de los embriones primarios, embriones tipo globular y embriones tipo cotiledonar.

embriogenseis Nevertheless, a high number of works report callus formation prior to the generation of the somatic embryos, even in the case of using tissues with embryogenic-predetermined cells zygotic embryosthereby increasing the risks of genetic variability in the plants obtained [5]. Zygotic embryogenesis and somatic embryogenesis from floral explants.

Agricultural and Food Science The role of activated charcoal in plant tissue culture. It is expected that these results could have a positive impact on so,atica propagation of this commercially relevant crop in Cuba in the short time, also setting guidelines for future research on the genetic regulation vegeta SE in papaya species. Factors affecting secondary somatic embryogenesis and embryo maturation in Morus alba L.

Por otro lado, Guiltinan et al.

Topicos de La Embriogenesis Somatica Vegetal : Florio De Real Sunshine :

Somatic embryogenesis in Eucalyptus. Tissue culture of cacao, coffee somagica oil palm. Investigadora y asesora en cultivo in vitro vegetal. Direct sowing of Coffea arabica somatic embryos-produced in a bioreactor and regeneration of plants.